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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha : |
18/09/2014 |
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Actualizado : |
25/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Generation and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Crop Science, 2012, v. 52, no. 4, p. 1572-1582. |
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ISSN : |
0011-183X |
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DOI : |
10.2135/cropsci2011.07.0374 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published July, 2012. |
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Contenido : |
ABSTRACT.
A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus.
© Crop Science Society of America. |
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Palabras claves : |
LEGUMINOSAS FORRAJERAS; MARCADORES MOLECULARES. |
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Thesagro : |
CULTIVOS; PLANTAS FORRAJERAS. |
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Asunto categoría : |
--
F01 Cultivo |
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Marc : |
LEADER 02262naa a2200277 a 4500
001 1050609
005 2019-11-25
008 2012 bl uuuu u00u1 u #d
022 $a0011-183X
024 7 $a10.2135/cropsci2011.07.0374$2DOI
100 1 $aCASTILLO, A.
245 $aGeneration and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus.$h[electronic resource]
260 $c2012
500 $aArticle history: Published July, 2012.
520 $aABSTRACT. A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus. © Crop Science Society of America.
650 $aCULTIVOS
650 $aPLANTAS FORRAJERAS
653 $aLEGUMINOSAS FORRAJERAS
653 $aMARCADORES MOLECULARES
700 1 $aREBUFFO, M.
700 1 $aDALLA RIZZA, M.
700 1 $aFOLLE, G.
700 1 $aSANTIÑAQUE, F.
700 1 $aBORSANI, O.
700 1 $aMONZA, J.
773 $tCrop Science, 2012$gv. 52, no. 4, p. 1572-1582.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
05/04/2022 |
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Actualizado : |
01/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
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Páginas : |
13 p. |
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DOI : |
10.3389/fpls.2022.804839 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
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Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
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Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500
001 1062960
005 2022-09-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2022.804839$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource]
260 $c2022
300 $a13 p.
500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario.
520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
653 $aDSRNA,
653 $aPEST CONTROL
653 $aRNA-SEQ
653 $aRNAI
653 $aSTINK BUG
653 $aVATPASE A,
700 1 $aFRESIA, P.
700 1 $aMURCHIO, S.
700 1 $aMUJICA, V.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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